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. 2020 Oct 10;22:832–845. doi: 10.1016/j.omtn.2020.10.004

Figure 2.

Figure 2

Identification of the Source of the Differentially Expressed miRNAs (DEMs) in Synovial Fluid

(A) The relative expression levels of miR-455, miR-210, and miR320c in synovial tissue and culture supernatant (n = 10 for KOA and n = 6 for LLA patients). (B) Under IL-1β treatment (5 ng/mL), the precursor levels of the three DEMs in chondrocytes and their mature levels in culture supernatant were determined by qRT-PCR. Healthy chondrocytes from LLA patients were used for the above experiments. (C and D) The regulatory effects of IL-1β (5 ng/mL) and TGF-β1 (10 ng/mL) on the expression of the three DEMs in synoviocytes and macrophages were estimated by qRT-PCR (C) and in situ hybridization (D). Synoviocytes and monocyte-derived macrophages from KOA patients were used for the above experiments. qRT-PCR data are presented as the mean and standard deviation (SD); U6 snRNA was detected as an endogenous control for miRNA in synovial tissue and cells. A representative image of in situ hybridization data is shown. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.