FIGURE 5.

(I) Scatter plot of the PCA of spectra corresponding to cytoplasm, nucleus and nucleolus of the (8 h) unexposed and PS-NH₂ exposed cells for 10 μM concentration of nanoparticles. Different cellular regions are coded as follows; red for cytoplasm, green for nucleus and blue for nucleolus. Exposed and unexposed cells are indicated by open circles and closed circles, respectively. (II) Mean difference spectra of cytoplasm (red), nucleus (green) and nucleolus (blue) obtained by subtraction of mean spectra of 8 h PS-NH₂ exposed cells from mean spectra of unexposed cells. The spectra are offset for clarity, the dashed line indicating the zero point. The bands related to PS are indicated with gray highlights (Efeoglu et al., 2016).