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. 2020 Oct 10;22:815–831. doi: 10.1016/j.omtn.2020.10.003

Figure 4.

Figure 4

miR-433-3p Is Vital for Cell Proliferation, Migration, and Invasion of Bladder Cancer Cells

(A) miR-433-3p levels were detected in bladder cancer cells (J82, 5637, T24, EJ, and TCCSUP) and SV-HUC-1 by qRT-PCR. (B) qRT-PCR indicated that miR-433-3p exhibited lower levels in bladder cancer tissues than in normal adjacent tissues (n = 20). (C) Pearson correlation analysis showing that expression levels of miR-433-3p are negatively correlated with circRIMS1 in 20 pairs of clinical specimens. Cells were transfected with miR-433-3p sponge and miR-433-3p and their negative controls, respectively. (D) Cell viability was measured by a CCK-8 assay to evaluate the biological effects of miR-433-3p. (E) Colony-formation assay indicated the vital role of miR-433-3p in cell proliferation. (F) Transwell migration and invasion assays indicated the role of miR-433-3p on cell migration and invasion. (G) Wound-healing assay showed that miR-433-3p influenced the cell migration ability. (H) Expression levels of MMP2, E-cadherin, N-cadherin, and vimentin in T24 and EJ cells transfected with pre-miR-433 or miR-433-3p sponge. ∗p < 0.05, ∗∗p < 0.01 versus control group.