Figure 7.

circRIMS1 Regulates Bladder Cancer Progression through Restoring the Expression of CCAR1

Cells under different conditions (blank control, sh-circRIMS1 negative control, CCAR1 overexpression negative control, circRIMS1 knockdown, CCAR1 overexpressing, or sh-circRIMS1 with CCAR1 overexpression) were used in the following assays. (A) Cell viability was evaluated to determine the effects on proliferation rate in T24 and EJ cells. (B) Colony-forming ability was detected by colony-formation assay. (C and D) Transwell migration and invasion assays (C) and wound-healing assays (D) indicated that CCAR1 overexpression promoted migration and invasion of circRIMS1-knockdown cells and inhibition of circRIMS1 attenuated CCAR1-induced biological effects. (E) Effects of sh-circRIMS1, CCAR1 overexpression, or sh-circRIMS1 with CCAR1 overexpression on expression levels of MMP2 and EMT-related genes were detected at the protein level. ∗p < 0.05, ∗∗p < 0.01 versus control group.