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. 2020 Oct 29;8:589468. doi: 10.3389/fbioe.2020.589468

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Copyright © 2020 Malcı, Walls and Rios-Solis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Wicket system allowing the integration of multiple pathway genes via universal homologs arms on the target genomic region. Pre-placed synthetic sequences act as gates for the integration of donor DNAs. It is possible to integrate pre-assembled multi genes of a metabolic pathway or to integrate a mix of each single donor DNA simultaneously. (B) Landing pad system providing controlled multiple integration of genes of interest. In this approach, a certain number of copies of pre-placed gates is present in the genome so that a particular copy number for each target gene or DNA fragment can be obtained.