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. 2020 Oct 29;11:595798. doi: 10.3389/fmicb.2020.595798

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Copyright © 2020 Marr, MacDonald, Trivedi, Chakravorty and Russo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Volcano plot of transcriptome analysis of AB307-0294 and its BfmR-deficient derivative AB307.70 (ΔbfmR). The ratio of RNA abundance in AB307-0294/AB307.70 (ΔbfmR) was calculated and plotted as log2-fold change. The dashed vertical lines left of zero marks a log2- fold change of -1 and the dashed line right of zero shows marks a log2 fold-change greater than 1, corresponding to a fold change of 0.5 and 2, respectively. A total of 3511 genes were assessed, those upregulated >10-fold are marked in blue, those downregulated >10-fold are marked in red, and those of interest for this study are marked in green, ABBFA_RS14200 (bfmS), ABBFA_RS01340 (mapA), ABBFA_RS03105 (oprB), and ABBFA_RS14205 (bfmR). The plot was generated using R package version 1.4.0, https://github.com/kevinblighe/EnhancedVolcano.