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. 2020 Oct 29;14:579466. doi: 10.3389/fnana.2020.579466

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Copyright © 2020 Stangerup and Hannibal.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Distribution of VPAC1 (green) and oxytocin (OXT; red) immunoreactivity in the mid and rostral part of SCN and PVN in a wild-type mouse. Images (A–F) represent maximal projections of 16 digital sections in a Z-stack (Z = 2 μm). (A,B) show immunoreactivity for VPAC1 in the rostral and mid part of PVN. (C,D) show immunoreactivity for OXT in the rostral and mid part of PVN. Colocalization between VPAC1 and OXT in the rostral and mid part of PVN is visualized in (E,F) using IMARIS colocalization module (see “Materials and Methods” section). Scale bars: (A–F) = 80 μm.