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. 2020 Oct 29;10:569584. doi: 10.3389/fonc.2020.569584

Figure 7.

Figure 7

LOXL2 regulated Atg7 expression by promoting Erk1/2 phosphorylation. (A) Protein levels of Erk1/2, p-Erk1/2, Atg7, epithelial-to-mesenchymal transition (EMT)-related markers, LC3-I, and LC3-II in differently transfected glioma cells. (B) Immunofluorescence of stubRFP-LC3 punctuate localization in U87 and U251 cells with EV, LOXL2, LOXL2 + PD98059, and LOXL2 + si-Erk1/2 treatment; scale bar = 40 µm (C) Immunofluorescence was used to assess Vimentin and N-cadherin expression in glioma cells under different transduction conditions; scale bar = 100 µm. (D) Wound healing and (E) Transwell assays of glioma cells for comparison of cell migration and invasion with EV, LOXL2, LOXL2 + PD98059, and LOXL2 + si-Erk1/2 treatment; scale bar = 100 µm. (F) Viability of glioma cells with EV, LOXL2, LOXL2 + PD98059, and LOXL2 + si-Erk1/2 with indicated TMZ doses. *P < 0.05; **P < 0.01, ns: P ≥ 0.05.