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. 2020 Nov 10;6(5):1423–1435. doi: 10.1016/j.bioactmat.2020.10.020

Fig. 3.

Fig. 3

In vitro cellular studies. (A) Confocal fluorescence images of HepG2, Bel-7402 and LO2 cells incubated with SAPEG-MPDA@SPIO/DOX/Fe3+ and PEG-MPDA@SPIO/DOX/Fe3+ for 0.5 and 4 h, respectively. Scale bar = 75 μm. (B) The fluorescence intensity of HepG2, Bel-7402 and LO2 cells treated with SAPEG-MPDA@SPIO/DOX/Fe3+ or PEG-MPDA@SPIO/DOX/Fe3+ NPs for 0.5 and 4, respectively. (C) Effect of NIR irradiation on the change of fluorescence intensity of HepG2, Bel-7402 and LO2 cells treated with SAPEG-MPDA@SPIO/DOX/Fe3+NPs. The T1 weighted image (D) and relatively T1 MR signal intensity (F) of HepG2, Bel-7402 and LO2 cells incubated with SAPEG-MPDA@SPIO/Fe3+ and PEG-MPDA@SPIO/Fe3+ NPs for 4 h, respectively. The T2-weighted image (E) and relatively T2 MR signal intensity (G) of HepG2, Bel-7402and LO2 cells incubated with SAPEG-MPDA@SPIO/Fe3+ and PEG-MPDA@SPIO/Fe3+ NPs for 4 h, respectively. Control is the cells without any treatment, (a) is the cells treated with PEG-MPDA@SPIO/Fe3+, and (b) is cells treated with SAPEG-MPDA@SPIO/Fe3+ NPs. (H) Cell viability of HepG2 cells treated with free DOX, SAPEG-MPDA@SPIO/DOX/Fe3+ and PEG-MPDA@SPIO/DOX/Fe3+ NPs at different DOX dosage. (I) Cell viability of HepG2 cells incubated with various concentrations of SAPEG-MPDA@SPIO/DOX/Fe3+ NPs with or without NIR irradiation. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n = 3).