Fig. 2.

Schematic representation of 2D PLS-DA scores plot and important features.

Metabolite features and concentrations from embryos (E+ and E−; n = 5 per group per time point per analysis) were normalized and assessed by partial least squares – discriminant analysis (PLSDA). 2D score plots were produced to separate feature differences between E+ and E− embryos at (A) 12-, (C) 24-, and (E) 48 hpf. E+ is shown in blue, E− is shown in red with corresponding 95% confidence regions (CR) drawn around each sample group. Variable importance of projection (VIP) scores from Component 1 of each PLS model indicate the most important features separating E+ and E− embryos at (B) 12-, (D) 24-, and (F) 48 hpf. Black and white boxes to the right of the VIP score plot indicate relative concentrations of each metabolite between E+ and E− embryos at the given time point.