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. 2020 Nov 4;38:101784. doi: 10.1016/j.redox.2020.101784

Fig. 4.

Fig. 4

Quantitative analysis of thiols, amino acids and related substances, Y-axes vary according to the concentration of each compound (pmol/embryo), X-axes shown times as 12, 24 and 48 hpf.

Embryos (E+, E− or control; n = 5 per group per time point per analysis) were analyzed either by the AA protocol (choline, betaine, SAM, SAH, methionine, serine and glutamate) and the thiol protocol (homocysteine, cysteine, cystine, GSH and GSSG). Data shown are pmol/embyro (mean ± SEM) at 12, 24 and 48 hpf. Symbols denote significant differences, as determined by Tukey post-hoc comparisons, at the time interval shown: E+ vs E− ‡P ≤ 0.05, ‡‡P ≤ 0.01, ‡‡‡P ≤ 0.001, ‡‡‡‡P ≤ 0.0001; control vs E− *P ≤ 0.05, **P ≤ 0.01, ****P ≤ 0.0001; control vs E+ #P ≤ 0.05, ##P ≤ 0.01, ###P ≤ 0.001. (A) Choline (Interaction, P = 0.0670, Time P < 0.0001, Group P = 0.7725), (B) Betaine (Interaction, P < 0.0001, Time P < 0.0001, Group P < 0.0001), (C) SAM (Interaction, P = 0.2115, Time P < 0.0001, Group P = 0.0666), (D) Homocysteine (Interaction, P = 0.3539, Time P = 0.0802, Group P = 0.4504), (E) Methionine (Interaction, P = 0.3661, Time P < 0.0001, Group P = 0.7944), (F) SAH (Interaction, P = 0.0405, Time P < 0.0001, Group P = 0.0271), (G) Serine (Interaction, P = 0.0357, Time P < 0.0001, Group P = 0.4039), (H) Cystine (Interaction, P = 0.7759, Time P < 0.0001, Group P < 0.0001), (I) Cysteine (Interaction, P = 0.1655, Time P < 0.0001, Group P = 0.2320), (J) GSH (Interaction, P < 0.0001, Time P < 0.0001, Group P < 0.0001), (K) Glutamate (Interaction, P = 0.2791, Time P < 0.0001, Group P = 0.0001), (L) GSSG (Interaction, P = 0.0977, Time P < 0.0001, Group P = 0.0622).