Table 1.
Embryo group | GSH1 | GSSG2 | GSH:GSSG3 |
---|---|---|---|
Control | |||
12 hpf | 569 ± 18 | 0.13 ± 0.01a | 4547 ± 432a |
24 hpf | 747 ± 37a | 0.30 ± 0.05a | 2678 ± 388 b |
48 hpf | 809 ± 37a | 1.14 ± 0.07 | 717 ± 52c |
E + l | |||
12 hpf | 454 ± 13 | 0.13 ± 0.01a | 3514 ± 317a |
24 hpf | 709 ± 27a | 0.18 ± 0.01a | 4113 ± 325a,b |
48 hpf | 656 ± 30a | 0.99 ± 0.09 | 690 ± 82c |
E–l | |||
12 hpf | 489 ± 9a | 0.12 ± 0.00a | 4185 ± 244a |
24 hpf | 695 ± 11b | 0.28 ± 0.04a | 2771 ± 475 b |
48 hpf | 529 ± 21a | 1.11 ± 0.11 | 489 ± 41c |
Concentrations are shown for GSH and GSSG (pmol/embryo, mean ± SEM, n=5 group per time).
1GSH: Interaction, P<0.0001; For the E+ vs E– comparisons: at 12 and 24 hpf, NS; 48 hpf, P<0.01. Within each group, values that do not share the same letter are different (P<0.001) at the indicated times.
2GSSG, time main effect P<0.0001. Within each group, values that do not share the same letter are different (P<0.001) at the indicated times
3GSH:GSSG (mol:mol ± SEM, n=5 per time point): interaction P=0.0045; values were significantly different (P<0.05) 12 vs 24, except NS for the E+ 12 and 24 hpf comparison; all other times within each group, P<0.001.