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. 2020 Nov 4;38:101784. doi: 10.1016/j.redox.2020.101784

Table 1.

GSH and GSSG concentrations (pmol/embryo) over time by embryo group.

Embryo group GSH1 GSSG2 GSH:GSSG3
Control
 12 hpf 569 ± 18 0.13 ± 0.01a 4547 ± 432a
 24 hpf 747 ± 37a 0.30 ± 0.05a 2678 ± 388 b
 48 hpf 809 ± 37a 1.14 ± 0.07 717 ± 52c
E + l
 12 hpf 454 ± 13 0.13 ± 0.01a 3514 ± 317a
 24 hpf 709 ± 27a 0.18 ± 0.01a 4113 ± 325a,b
 48 hpf 656 ± 30a 0.99 ± 0.09 690 ± 82c
E–l
 12 hpf 489 ± 9a 0.12 ± 0.00a 4185 ± 244a
 24 hpf 695 ± 11b 0.28 ± 0.04a 2771 ± 475 b
 48 hpf 529 ± 21a 1.11 ± 0.11 489 ± 41c

Concentrations are shown for GSH and GSSG (pmol/embryo, mean ± SEM, n=5 group per time).

1GSH: Interaction, P<0.0001; For the E+ vs E– comparisons: at 12 and 24 hpf, NS; 48 hpf, P<0.01. Within each group, values that do not share the same letter are different (P<0.001) at the indicated times.

2GSSG, time main effect P<0.0001. Within each group, values that do not share the same letter are different (P<0.001) at the indicated times

3GSH:GSSG (mol:mol ± SEM, n=5 per time point): interaction P=0.0045; values were significantly different (P<0.05) 12 vs 24, except NS for the E+ 12 and 24 hpf comparison; all other times within each group, P<0.001.