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. 2020 Oct 29;10:581364. doi: 10.3389/fonc.2020.581364

Figure 2.

Figure 2

Sustained exposure to H. pylori lysate alters migration and invasion of GES-1 and MKN-45 cells. GES-1 or MKN-45 cells were treated with H. pylori lysate (2 μg/mL for GES-1 cells and 1.5 μg/mL for MKN-45 cells) or H. pylori (6×106 CFU/mL) for 24 h. (A) The wound healing assay was used to determine the migration of Cul30-GES-1 and B-GES-1 cells exposure to H. pylori lysate or H. pylori. Red lines represent the borders of the wounds (n=5 experiments). (B–E) Transwell migration (B, C) and Transwell invasion (D, E) assays were performed to detect the migration and invasion ability of Cul30-GES-1, B-GES-1, Cul30-MKN-45, and B-MKN-45 cells exposed to H. pylori lysate or H. pylori. (F–K) Expression of CCL-20 (F), CCL-28 (G), and CXCL-2 (H) in GES-1 cells, and the expression of CCL-20 (I), CCL-28 (J), and CXCL-2 (K) in MKN-45 cells were measured by Luminex assays. n = 3. *p < 0.05, **p < 0.01.