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. 2020 Oct 29;13:574947. doi: 10.3389/fnmol.2020.574947

Figure 1.

Figure 1

Changes in the expression of cytoplasmic fragile X mental retardation 1 (FMR1)-interacting protein 1 (CYFIP1), CYFIP2, and Wiskott–Aldrich syndrome protein family verprolin-homologous protein 1 (WAVE1) in multiple brain regions of Cyfip2 conditional knock-out (cKO) mice. (A) The breeding scheme for the Cyfip2 cKO mice. (B) Real-time quantitative reverse transcription PCR (qRT-PCR) analyses of Cyfip1, Cyfip2, and Wasf1 (encoding WAVE1) mRNAs in the cortex (left panel) and cerebellum (right panel) of Cyfip2 cKO mice (n = 4 animals per genotype, one-way analysis of variance (ANOVA) with Bonferroni’s post hoc test). NS, not significant. (C–F) Western blot analysis of CYFIP1, CYFIP2, and WAVE1 proteins in the cortex, striatum, hippocampus, and cerebellum of Cyfip2 cKO mice (n = 4 animals per genotype, one-way ANOVA with Bonferroni’s post hoc test). All data are presented as mean ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001.