Figure 3.

Detection of Partial AAV Genomes and Genomes from Different Vectors

(A) Low and high magnification images of the 5′ (AAV-5′, green) and 3′ (AAV-3′, red) halves of the AAV8-CMV-GFP genome in retinas at 24 h or 2 weeks after subretinal injection of ∼2.5 × 10⁸ vg of AAV8-CMV-GFP. Colocalized green and red puncta appear yellow. Scale bars, 20 μm. (B) Low and high magnification images of the AAV8-Best1-sCX3CL1 (AAV-A, green) and AAV8-CMV-GFP (AAV-B, red) genomes in retinas at 24 h or 2 weeks after subretinal injection of ∼2.5 × 10⁸ vg of each vector. Scale bars, 20 μm. (C) Probability of green and red colocalization for the combinations tested in (A) and (B), defined as the fraction of puncta in the retina that colocalized with puncta of the other color. n = 3–5 animals per group. Data shown are mean ± SEM. ∗∗∗∗p < 0.0001 by two-tailed Student’s t test.