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. 2020 Oct 14;22:885–899. doi: 10.1016/j.omtn.2020.10.007

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© 2020 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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The Smad-Targeting siRNAs Effectively Inhibit BMP9-Induced Early Osteogenic Marker Alkaline Phosphatase (ALP) Activity in BMSCs

The BMSCs stably expressing simSmad4, simSmad1458, simSmad4158 or siControl cells (i.e., BMSCs) were infected with AdGFP or AdBMP9. (A and B) ALP activity was histochemically stained (A) and quantitatively assessed (B) at day 3 (a), day 5, and day 7(b). ALP activity was quantitatively determined by using a modified Great Escape SEAP chemiluminescence assay. Representative results are shown. ∗∗p < 0.01, compared with that of the siControl counterpart (BMSCs).