Figure 1.

Time Course of Folic-Acid-Induced Kidney Injury and miR-150 Expression in Mice

(A–C) Evaluation of kidney injury after the injection of folic acid (FA). Shown are (A) the ratio of kidney weight to heart weight, (B) serum creatinine (Scr), (C) blood urea nitrogen (BUN), and (D) PAS staining. Magnification 400×, scale bar, 10 μm. Black asterisks indicate tubular ectasia, loss of brush border, flattened or detached epithelial cells; yellow arrows indicate infiltration of inflammatory cells in tubulointerstitium; white arrows indicate patchy fibrosis. (E and F) miR-150 expression assessed by (E) fluorescence in situ hybridization (FISH) and (F) qPCR. Magnification 200×, scale bar, 400 μm; n = 6, # p < 0.05, day 2, day 7, day 30 versus day 0; ∗p < 0.05, day 7, day 30 versus day 2. Data represent mean ± SD. See also Figure S4A.