Figure 6.

The Effect of miR-150 Inhibitor on SOCS1/JAK/STAT Pathway in HK-2 Cells

(A) miR-150 (green), SOCS1 protein (red), and co-expression (yellow) in untreated HK-2 cells assessed by double staining using FISH and immunofluorescence (magnification 400×, scale bar, 20 μm). (B) qPCR of miR-150 in HK-2 cells co-cultured with macrophages. (C) SOCS1 mRNA expression. (D) mRNA levels of pro-fibrotic genes including α-SMA, FN, and COL-1. (E) Western blotting for SOCS1, p-JAK1, p-JAK2, p-STAT1, p-STAT3, α-SMA, FN, and COL-1 (n = 3, # p < 0.05, HK-2+ macrophages versus HK-2 cells. ∗p < 0.05, siRNA of miR-150 versus scrambled negative control (NC) oligonucleotides. Data represent mean ± SD. See also Figure S4C.