TABLE 2.

Summary of the results obtained for rec-PrP^res propagation in PMSA from the different prion-coated beads treated with the chosen different decontamination procedures.

	Autoclave 121°C		Autoclave 134°C		UV		NaOH		Bleach		VirkonTM		AcO-SDS
Material	t2	t3	t2	t3	t2	t3	t2	t3	t2	t3	t2	t3	t2	t3
Glass	6/6	6/6	0/6∼	4/6 (±0.52)	6/6	6/6	4/6 (±0.52)	6/6	0/6∼	2/6∼ (±0.52)	3/6× (±0.55)	6/6	2/6∼ (±0.52)	6/6
Zr-Si	6/6	6/6	5/6 (±0.41)	6/6	5/6 (±0.41)	6/6	3/6× (±0.55)	6/6	0/6∼	0/6∼	5/6 (±0.41)	6/6	5/6 (±0.41)	6/6
Steel	0/6∼	0/6∼	0/6∼	0/6∼	5/6 (±0.41)	6/6	0/6∼	0/6∼	0/6∼	0/6∼	2/6 (±0.52)	6/6	4/6 (±0.52)	6/6
Teflon®	3/6 (±0.55)	6/6	0/6∼ (±0.55)	2/6∼ (±0.52)	3/6 (±0.55)	6/6	3/6 (±0.55)	6/6	1/6× (±0.41)	5/6 (±0.41)	0/6∼	0/6∼	3/6 (±0.55)	6/6

Number of replicate tubes subjected to PMSA showing rec-PrP^res by electrophoresis and total protein staining/total number of replicates. Presence of rec-PrP^res indicates incomplete inactivation of the rec-PrP^res adsorbed to the surface of the beads. t2 refers to the shortest PMSA reaction time in which rec-PrP^res was detected in the absence of any decontamination treatment but propagation has not reach a plateau, and t3 refers to the shortest PMSA reaction time in which rec-PrP^res signal was considered to have reached plateau which is considered to be the maximum propagation limit. The numbers in parenthesis show the mean ± SD, excluding those cases for which SD was 0. Statistically significant differences with respect to untreated controls are also indicated with the following symbols: ^×p < 0.05, ^××p < 0.01, and ^∼p < 0.001.