TABLE 3.
Summary of the results obtained for rec-PrPres propagation in PMSA from the prion-coated Teflon® beads treated with NaOH and bleach with Triton X-100 added.
| NaOH | NaOH + Triton X-100 | Bleach | Bleach + Triton X-100 | |||||
| Material | t2 | t3 | t2 | t3 | t2 | t3 | t2 | t3 |
| Teflon® | 3/6 (± 0.55) | 6/6 | 0/6∼ | 0/6∼ | 1/6× (±0.41) | 5/6 (±0.41) | 0/6∼ | 0/6∼ |
Number of replicate tubes subjected to PMSA showing rec-PrPres by electrophoresis and total protein staining/total number of replicate tubes is represented for each treatment. Presence of rec-PrPres indicates incomplete inactivation of the seeds adsorbed to the surface of the beads. t2 refers to the shortest PMSA reaction time in which rec-PrPres can be detected in the absence of a decontamination treatment but propagation has not reach a plateau, and t3 refers to the shortest PMSA reaction time in which the rec-PrPres signal reached plateau, which is considered to be the maximum propagation limit. The numbers in parenthesis show the mean ± SD, excluding those cases for which SD was 0. Statistically significant differences with respect to untreated controls are also indicated with the following symbols: ×p < 0.05, ××p < 0.01, and ∼p < 0.001.