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. 2020 Oct 29;8:570528. doi: 10.3389/fchem.2020.570528

Scheme 1.

Scheme 1

Comparison of aptamer- and antibody-based protein blotting analysis. After common electrophoresis, transfer and blocking steps, fluorescently labeled aptamers can be directly applied for selective protein recognition, while conventional Western blot requires two incubation steps with primary and secondary antibodies, followed by a chromogenic reaction and chemiluminescence detection.