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Centrinone-treated RPE1 cells expressing in situ mNeonGreen-tagged CEP192 and transgene-encoded mCherry-fused microtubule-binding domain of MAP4. One-centrosome and zero-centrosome cells are shown in control RNAi versus CDK5RAP2 RNAi. 8 × 2–µm z-stacks were acquired every 5 min at 40× magnification. Image frames shown are maximum intensity projections at each time point. Playback is sped up 900× relative to real-time. Elapsed time shown is in hours:minutes, and scale bar is 10 µm.
