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. 2020 Oct 23;23(11):101726. doi: 10.1016/j.isci.2020.101726

Figure 3.

Figure 3

Concomitant Deletion of Region 5 Increases the Severity of the Inflammatory Phenotype Due to the Removal of the ARE from Tnf 3′ UTR

(A–C) Esophagitis (A), ileitis (B), and colitis (C) in a 100 day-old Tnfdel4del5/+ mouse. Arrowheads indicate immune cell infiltration in these tissues.

(D) Homozygote Tnfdel4del5/del4del5 (m/m) embryos die at E16.5 and appear devoid of major blood vessels.

(E–I) (E) Active Caspase-3 staining of an E15.5 homozygote TNFdel4del5 embryo (m/m) shows extensive cell death in the liver. Active Caspase-3-stained liver of WT (F) and homozygote (G) E16.5 TNFdel4del5 embryos at 40× magnification. 20× magnification shows death of blood vessels in the digits of an E16.5 TNFdel4del5 homozygote embryo (I) and the absence of cell death in WT embryo (H) at the same stage of development.

(J and K) Label-free light sheet imaging of hemoglobin autofluorescence in cleared front paws of WT (+/+) and homozygote Tnfdel4del5/del4del5 (m/m) E16.5 embryos. Still image from Video S1.

See also Figures S3, S4 and S5.