Fig. 3.

Cardiac-specific deletion of PGAM5 reduces myocardial infarction area and attenuates the inflammatory response. (A–C). Assessment of area at risk (AAR) and infarct size (IS) using Evans blue and TTC staining, respectively, following 45 min ischemia/6 h reperfusion. Percentual IS and AAR values were multiplied by the corresponding section weight. IS/AAR are presented as percentages. (D–F) ELISA analysis of serum markers of cardiac damage. (G) Representative electron microscopy images of reperfused heart samples. White arrowheads indicate mitochondrial swelling and red arrowheads indicate Z-line disappearance. (H–I) Transcriptional analysis of pro-inflammatory gene expression in cardiomyocytes exposed to sI/R. Experiments were repeated three times with similar results. Data are shown as the means ± SEM, n = 3 independent cell isolations per group. *p < 0.05. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)