Table 2.
Assay Precision Evaluated by Ct Values Obtained From Real-Time Quantitative PCR Analysis of Standard EBV DNA.
| Level | Average observed EBV DNA titer (log10 copies/mL) | Within-run precision | Between-run precision | Total precision | |||
|---|---|---|---|---|---|---|---|
| SD | CV (%) | SD | CV (%) | SD | CV (%) | ||
| 1 | 6.079 | 0.081 | 0% | 0.277 | 1% | 0.231 | 1% |
| 2 | 5.079 | 0.081 | 0% | 0.192 | 1% | 0.166 | 1% |
| 3 | 4.380 | 0.195 | 1% | 0.287 | 1% | 0.263 | 1% |
| 4 | 4.079 | 0.170 | 1% | 0.658 | 2% | 0.594 | 2% |
| 5 | 3.778 | 0.918 | 3% | 0.488 | 1% | 0.766 | 2% |
Abbreviation: CV, coefficient of variation; EBV, Epstein Barr virus; PCR, polymerase chain reaction.