REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
CD3ε (145-2C11)	TONBO Biosciences	Cat#65-0031-U100; RRID: AB_2810847
Ly-6G (RB6-(C5)	BioLegend	Cat#127623; RRID: AB_10645331
sca-1 (E13-161.7)	BioLegend	Cat#108128; RRID: AB_2563064
Lineage Cocktail antibody	BD Biosciences	Cat#559971; RRID: AB_10053179
c-kit (2B8)	eBioscience	Cat#11-1171-81; RRID: AB_465185
CD150 (TC15-12F12.2)	BioLegend	Cat#115927; RRID: AB_11204248
Brilliant Violet 510™ Streptavidin	BioLegend	Cat#405233; RRID: AB_2810848
CD45.1 (A20)	BD Biosciences	Cat#560578; RRID: AB_1727488
CD45.2 (104)	TONBO Biosciences	Cat#20-0454; RRID: AB_2621576
Alexa Fluor 488 Sreptavidin-conjugated antibody	Invitrogen	Cat#S32354; RRID: AB_2315383
Mouse Anti-Human CD34  Clone 581 (RUO)	BD Biosciences	Cat# 555824, RRID: AB_398614
B220 (RA3-6B2)	BD Biosciences	Cat#553088; RRID: AB_394618
CD11b (M1/70)	BioLegend	Cat#101208; RRID: AB_312791
Biotin Rat Anti-Mouse CD31 Clone MEC 13.3	BD Biosciences	Cat#553371; RRID: AB_394817
Biotin Rat Anti-Mouse TER-119/Erythroid Cells  Clone TER-119 (RUO)	BD Biosciences	Cat#553672; RRID: AB_394985
Biological Samples
Human acute myeloid leukemia specimens	Cambridge University Hospitals, Cambridge, UK	REC 07-MRE05-44
Human acute myeloid leukemia specimens	University Hospital Sant’Orsola-Malpighi, Bologna, Italy	94/2016/O/Tess
Chemicals, Peptides, and Recombinant Proteins
Fetal Bovine Serum, charcoal stripped	GIBCO	Cat#12676029
Cytarabine	Cayman Chemical	Cat#CAY16069
Collagenase Type I (0.25%)	Stem Cell Technologies	Cat#07902
Doxorubicin	Sigma	Cat#D1515
Lympholyte®-M Cell Separation Media	Cedarlane	Cat#CL5031
DMEM, high glucose, GlutaMAX™ Supplement, pyruvate	Thermo Fisher Scientific	Cat#31966021
RPMI Medium 1640 (1X) no phenol red	Thermo Fisher Scientific	Cat#11835-063
Murine IL-6	PeproTech	Cat#216-16-50
Murine SCF	PeproTech	Cat#216-16-50
Murine IL3	PeproTech	Cat#213-13-100
human PDGF-AA	PeproTech	Cat#100-13A-100
Human Oncostatin M (227 a.a.)	PeproTech	Cat#300-10T
Human IL-6	PeproTech	Cat#200-06-50
Human FGF-basic (154 a.a.)	PeproTech	Cat#100-18B
Poly(2-hydroxyethyl methacrylate)	Sigma-Aldrich	Cat#P3932
Human EGF	PeproTech	Cat#AF-100-15
Human IGF-I	PeproTech	Cat#100-11
B-27 Supplement (50X), serum free-10 mL	N/A	N/A
N-2 Supplement (100X)-5 mL	N/A	N/A
Trypsin (0.25%), phenol red	Gibco	Cat#15050065
DOXYCYCLINE HYCLATE, =98% (HPLC)	Sigma-Aldrich	Cat#D9891-25G
Penicillin-Streptomycin	Thermo Fisher Scientific	Cat#15140122
Corning™ Cell-Tak Cell and Tissue Adhesive	Fisher Scientific Ltd	Cat#CB40240
Dihydrorhodamine 123	Invitrogen	Cat#D23806
BODIPY™ 581/591 C11 (Lipid Peroxidation Sensor)	Invitrogen	Cat#D3861
Monochlorobimane (mBCI)	Invitrogen	Cat#M1381MP
MitoTracker™ Red CMXRos	Invitrogen	Cat#M7512
MitoTracker™ Green FM	Invitrogen	Cat#M7514
DAPI	Sigma-Aldrich	Cat#9542
Mercaptosuccinic acid	Sigma-Aldrich	Cat#M6182
DMSO	Sigma-Aldrich	Cat#D5879
buthionine sulfoximine (BSO)	N/A	Cat#NSC 326231
DTAB	Sigma-Aldrich	Cat# D8638
0.5M Trizma® base	Sigma-Aldrich	Cat#T1503
Critical Commercial Assays
XF Cell Mito Stress Test	Agilent Technologies	Cat#103015-100
Seahorse XF Glycolytic Rate Assay	Agilent Technologies	Cat#103344-100
Pacific Blue™ Annexin V/SYTOX™ AADvanced™ Apoptosis Kit	Invitrogen	Cat#A35136
High-Capacity cDNA Reverse Transcription kit	Applied Biosystems	Cat#4368814
PowerUp SYBR Green Master Mix	Applied Biosystems	Cat#A25742
EasySep™ Human CD271 Positive Selection Kit	StemCell technologies	Cat#17849
ABC amplification kit	Vector Labs	Cat#AK-5000
Dynabeads mRNA DIRECT Kit	Thermo Fisher Scientific	Cat#61012
NADP/NADPH Glo Assay	Promega	Cat#G9081
Clit iT protein enrichment kit	Thermo Fisher Scientific (Life Technologies)	Cat#C10416
Arcturus Picopure RNA isolation kit	Applied Biosystems	Cat#KIT0204
Deposited Data
TCGA dataset	cBioPortal (Cerami et al., 2012)	(Cancer Genome Atlas Research et al., 2013)
Proteomics data derived from this study	This study	Peptide Atlas (http://www.peptideatlas.org/repository/)Accession code: PASS01476
RNAseq data from nestin+ BMSCs obtained from leukemic iMLL-AF9 and normal mice	This study	Gene Expression Omnibus (GEO), GSE140135
RNAseq data from leukemic BM lin-ckitlo cells obtained from mice with/without nestin+ cell depletion	This study	Gene Expression Omnibus (GEO), GSE140207
Experimental Models: Cell Lines
bEnd.3 [BEND3]	ATCC	Cat#CRL-2299
Experimental Models: Organisms/Strains
congenic CD45.1 C57BL/6 mice	Charles River Laboratories	N/A
congenic CD45.2 C57BL/6 mice	Charles River Laboratories	N/A
rtTA;MLL-AF9 mice		Stavropoulou et al., 2016
Nes-creERT2 mice	Gift from G. Fishell	Balordi and Fishell, 2007
Nes-GFP mice	Gift from G. Enikolopov	Mignone et al., 2004
R26lacZbpAfloxDTA mice	Gift from D. Riethmacher	Brockschnieder et al., 2006
Oligonucleotides
mouse Gapdh forward: GCATGGCCTTCCGTGTTC, reverse: CTGCTTCACCACCTTCTTGAT	Sigma-Aldrich	N/A
mouse Gclc forward: AGGCTCTCTGCACCATCACT, reverse: CTCTGGGTTGGGTCTGTGTT	Sigma-Aldrich	N/A
mouse Gsr forward: GCTATGCAACATTCGCAGATG, reverse: AGCGGTAAACTTTTTCCCATT	Sigma-Aldrich	N/A
mouse Gpx1 forward: GGTTCGAGCCCAATTTTACA, reverse: CATTCCGCAGGAAGGTAAAG	Sigma-Aldrich	N/A
mouse Gpx3 forward: ACAATTGTCCCAGTGTGTGCAT, reverse: TGGACCATCCCTGGGTTTC	Sigma-Aldrich	N/A
mouse Actb forward: TGGCGCTTTTGACTCAGGAT, reverse: GGGATGTTTGCTCCAACCAA	Sigma-Aldrich	N/A
mouse Trx1 forward: ATGGTGAAGCTGATCGAGAGC, reverse: GGCATATTCAGTAATAGAGGC	Sigma-Aldrich	N/A
mouse Trx2 forward: GCTAGAGAAGATGGTCGCCAAGCAGCA, reverse: TCCTCGTCCTTGATCCCCACAAACTTG	Sigma-Aldrich	N/A
mouse Txnrd2 forward: GCTGGGCCTGCACTTCCT, reverse: ATGCACAGGTGATGCAGACAG	Sigma-Aldrich	N/A
mouse Txnrd1 forward: CAAATTTGACAAGAAAGTGCTG, reverse: AGTCATGCTTCACTGTGTCTTC	Sigma-Aldrich	N/A
mouse Sod1 forward: GTGATTGGGATTGCGCAGTA, reverse: TGGTTTGAGGGTAGCAGATGAGT	Sigma-Aldrich	N/A
mouse Sod2 forward: TTAACGCGCAGATCATGCA, reverse: GGTGGCGTTGAGATTGTTCA	Sigma-Aldrich	N/A
Software and Algorithms
Kaluza Analysis Software	Beckman Coulter	GalliosTM Kaluza, RRID: SCR_016700
GraphPad Prism v.8	GraphPad Software	GraphPad Prism, RRID: SCR_002798
Seahorse Wave v.2.2.0	Agilent	Seahorse Wave, RRID: SCR_014526
FlowJo	BD Biosciences	FlowJo, RRID: SCR_008520
Thermo Xcalibur	Thermo Fisher Scientific	Thermo Xcalibur, RRID: SCR_014593
TopHat v2.1.0	Johns Hopkins University, University of Washington and University of Maryland	TopHat, RRID: SCR_013035
R Project for Statistical Computing	R Foundation	R Project for Statistical Computing, RRID:SCR_001905
Proteome Discoverer v.2.1.0.81	Thermo Fisher Scientific	Proteome Discoverer, RRID: SCR_014477