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. 2020 Nov 11;10:19521. doi: 10.1038/s41598-020-76593-2

Figure 4.

Figure 4

Loss of KLB in glutamatergic neurons in lean mice blocks the effects on body weight, but not insulin sensitivity associated with dietary protein restriction. (A) Body weight curves of wild-type (WT) mice and mice lacking β-klotho (KLB) in Vglut2-expressing cells (KLB Vglut2-KO mice) on normal chow (NC) or low protein chow (LP) over 5 weeks (n = 9–10 mice/group). (B) Average weekly food intake of WT and KLB Vglut2-KO mice on NC or LP. (C,D) Percentage of fat mass (C) and lean mass (D) in WT and KLB Vglut2-KO mice after 5 weeks on NC or LP (n = 5 mice for each group). (E) Relative Fgf21 mRNA levels in the liver of WT and KLB Vglut2-KO mice following 5 weeks on NC or LP (n = 8–9 mice/group). (F) Relative Ucp1 mRNA expression in inguinal white adipose tissue (iWAT) of WT and KLB Vglut2-KO mice on NC or LP (n = 9 mice/group). (G) Total energy expenditure (EE) in WT and KLB Vglut2-KO mice on NC or LP determined by indirect calorimetry (n = 8 mice/group). (H) Plasma glucose levels during an insulin tolerance test (ITT) in WT and KLB Vglut2-KO mice on NC or LP (n = 6 mice/group). (I) Quantification of the average area above the curve for the ITT curves plotted in (H). Values are mean ± SEM. 2-way ANOVA with Holm-Sidak’s multiple comparisons test performed for all panels with mice on NC used as the control condition within genotypes for statistical comparisons, * = P < 0.05.