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. 2020 Nov 11;11(11):969. doi: 10.1038/s41419-020-03148-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A NSCLC cells were treated with different concentrations of gefitinib for 24 h. The inhibition rate of drugs in NSCLC cells was determined by CCK-8. B NSCLC cells were treated with gefitinib for 24 h, stained with Annexin V-FITC and PI, and detected by flow cytometry. C Western blotting was performed to study the expression of cleaved caspase-3 and cleaved PARP in NSCLC cells treated with indicated drugs. D Representative results of transwell analysis. The pictures were taken 24 h after the boarding (picture display magnification: ×200). E Representative results of wound healing assay. Pictures were taken every 24 h at the indicated time (picture display magnification: ×40). Cleaved Cas-3, Cleaved caspase-3; Con, control; Ge, gefitinib. The data were presented as mean ± SD. Asterisk indicates statistically significant difference (***p < 0.001, **p < 0.01, *p < 0.05).