Fig. 2. β-elemene could reverse gefitinib resistance in NSCLC.
A Representative pictures of colony-formation assay. NSCLC cells were treated with indicated concentration of drugs for about two weeks. B NSCLC cells were treated with indicated drugs for 24 h, and detected by flow cytometry after Annexin V-FITC/PI dual staining. C Effect of indicated drugs on tumor growth in nude mice and quantitative analysis of tumor volume. D PC9GR cells were treated with indicated drugs for 24 h. iTARQ sequencing was used to determine the protein expression of treated cells. Con, control; Ge, gefitinib; β-Ele, β-elemene. The data were presented as mean ± SD. Asterisk indicates statistically significant difference (***p < 0.001, **p < 0.01, *p < 0.05).