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. 2020 Nov 11;11(11):969. doi: 10.1038/s41419-020-03148-8

Fig. 3. β-elemene could reverse gefitinib resistance by inhibiting autophagy.

Fig. 3

A PC9GR and HCC827GR cells were treated with β-elemene for 24 h in basic and complete medium, the apoptosis rate was measured by flow cytometry after Annexin V-FITC/PI dual staining. B Gefitinib-resistant cells were treated with different drugs for 24 h. The inhibition rate of cells was determined by CCK-8. C Gefitinib-resistant cells were treated with different drugs for 24 h, the apoptotic cells were measured by flow cytometry after Annexin V-FITC/PI dual staining. D The apoptotic cells were measured by flow cytometry after Annexin V-FITC/PI dual staining after changing the expression of ATG5 or ATG7 in gefitinib-resistant cells. E Analysis of autophagy-related protein expression of cells threated with various drugs by western blotting. Con, control; Ge, gefitinib; β-Ele, β-elemene; 3-MA, 3-Methyladenine; CQ, chloroquine; Rapa, rapamycin; BFA, brefeldin A; NC, negative control; si, siRNA; OE, overexpression. The data were presented as mean ± SD. Asterisk indicates statistically significant difference (**p < 0.01, *p < 0.05).