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. 2020 Nov 11;11(11):969. doi: 10.1038/s41419-020-03148-8

Fig. 4. β-elemene blocked autophagy flux in NSCLC cells.

Fig. 4

A NSCLC cells were infected with GFP-RFP-LC3, and then treated with indicated autophagy-related drugs for further 24 h. The representative pictures of fluorescent LC3 spots were shown. Scale bar = 10 μm. B PC9GR cells were treated with β-elemene and chloroquine for 24 h, followed by a series of treatments and observed by transmission electron microscopy. Scale bar = 500 nm. C PC9GR and HCC827GR cells were treated with β-elemene and chloroquine for 24 h, stained with lyso-tracker red dye, and observed by fluorescence microscopy. Scale bar = 200 μm. D Effect of β-elemene and chloroquine on tumor growth in nude mice and quantitative analysis of tumor volume. E Immunohistochemical analysis of the expression of LC3B and SQSTM1 in tumor tissues harvested from nude mice with indicated drugs treatment. Con, control; β-Ele, β-elemene; CQ, chloroquine. The data were presented as mean ± SD. Asterisk indicates statistically significant difference (**p < 0.01, *p < 0.05).