Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 11;11:5716. doi: 10.1038/s41467-020-19513-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a To simulate low quality picking, only 10% of the initial crYOLO picks were used while the remaining 90% were re-positioned randomly (left). After the feedback loop crYOLO reliably picks the TRPC4 particles (right). b Total amount of 2D class averages produced in the first iteration of the feedback loop (top) and 21 representative averages produced in the final iteration of the feedback loop (bottom). c Progression of the number of particles labeled “good” when applying the intermediate picking models of the feedback loop to a fixed subset of 500 micrographs. The curve flattens out in the final iterations, indicating the convergence of the feedback loop optimization. d Fourier shell correlation (FSC) curves of the individual 3D reconstructions computed from particles labeled “good” (also see c). e Representative α-helix (amino acids 600–615) illustrating the improvement of the density when using the final (bottom) compared to the initial (top) picking model. f 3D reconstruction of TRPC4 computed from 500 micrographs using the optimized picking model.