Table 5.
Summary of the feedback loop statistics for the actomyosin complex.
Feedback round | Good classes | Good particles | Picks/Mic | Good picks/Mic | Resolution | Relative good picks |
---|---|---|---|---|---|---|
1 | 211 | 9629 | 143 | 96 | 7.63 | 0.67 |
2 | 659 | 29,559 | 358 | 296 | 4.48 | 0.83 |
3 | 936 | 42,779 | 552 | 428 | 4.37 | 0.77 |
4 | 1016 | 45,849 | 639 | 458 | 4.54 | 0.72 |
5 | 1203 | 54,134 | 1098 | 541 | 4.32 | 0.49 |
6 | 1174 | 53,221 | 1073 | 532 | 4.72 | 0.50 |
6 + T 0.3 | 869 | 38,946 | 515 | 389 | 4.54 | 0.76 |
For every feedback round as well as the final run after optimization of the picking threshold (6 + T x.xx) the number of classes labeled “good” by Cinderella; the number of particles included in these classes; the total number and the number of good particles picked per micrograph; the final resolution of the 3D reconstruction; and the relative amount of good particles (highlighted in bold) are listed for 100 micrographs.