Figure 2.

(A) 2D-¹H¹⁵N-BEST-TROSY and (B) ¹³C-detected CON spectra  acquired on the full-length NiV PNT. The 2D CON spectrum is much more resolved and enables also the direct detection of the correlations involving the 26 prolines in a clean region of the spectrum. As an example of the importance of those residues, the cross peaks of the residues in a SLiM are reported. The complete assignment, including the ¹³C nuclei, is reported in Supplementary Table S2.