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. 2020 Nov 11;10:19570. doi: 10.1038/s41598-020-76172-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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OCI-LY19 cells were treated during 64 h with the active (–SH) form of bromelain, papain and ananain (1 µM) or vehicle only. Live cell imaging was used to obtain pictures of the different culture conditions (a) and calculate the total cell surface (b) and the normalized SYTOX Green (SG) positive cell surface (c). Pictures were taken after 64 h of treatment. Arrows indicate dead cells. Scale bar = 200 µm. Data are presented as mean ± SEM (n = 3–4). Kolmogorov–Smirnov test: NS, not significant; ***p < 0.001; ****p < 0.0001.