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. 2020 Nov 11;10:19569. doi: 10.1038/s41598-020-76287-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of morphine and Kiss1 administration on kiss1 mRNA and 5-HT levels in the brain. Graph showing the kiss1 mRNA (A) and 5-HT levels (B) in different treated groups (n = 4–5 per group). Data are presented as mean ± SEM and analysed using one way ANOVA followed by Tukey’s multiple comparison test. *P < 0.05; **P < 0.01, ***P < 0.001.