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. 2020 Nov 11;10:19500. doi: 10.1038/s41598-020-76441-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Unbiased stereological quantification of F2-C6-D PVG rats at 42 days. (a) Representative image of myelin basic protein staining near epicenter of injury in an F2-C6-D rat. (b) Representative image of GFAP staining near epicenter of injury in an F2-C6-D rat. (c) Stereological assessment demonstrated a significant increase in % spared tissue in H-C6-D rats (Student’s t test, *p < 0.05). (d) However, in F2-C6-D rats showed no difference in % spared tissue (Student’s t test, p = 0.4). (e) No significant differences were measured in central lesion volume in H-C6-D rats (Student’s t test, p > 0.05). (f) A strong trend for increased central lesion volume in F2-C6-D rats (Student’s t test, *p = 0.08). Data points represent group means ± SEM. Scale bar 500 μm.