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. 2020 Nov 12;19:208. doi: 10.1186/s12934-020-01456-4

Fig. 2.

Fig. 2

Constructs of relevant plasmids. Arrows labeled “stacked” represent TEF1-HYG constructs that were transformed into the highest respective sesquiterpene-producing nourseothricin-resistant (NAT) strains to increase transgene expression. Rt represents R. toruloides codon optimized genes while Sc and Lp represent native genes from S. coelicolor and L. pacificia. NAT and HYG represent expression cassettes conferring resistance to nourseothricin and hygromycin B, respectively, and NOS represents the nopaline synthase terminator