Table 2.
Plasmids | Genotypes/features | Source/references | JBEI registry ID |
---|---|---|---|
Construct 1 | PGAPDH-ScEIZS-NATR | This study | JPUB_013517 |
Construct 2 | PGAPDH-RtEIZS-NATR | This study | JPUB_013519 |
Construct 3 | PTEF1-RtEIZS-HYGR | This study | JPUB_013521 |
Construct 4 | PANT-RtEIZS-PTEF1-RtEIZS-NATR | This study | JPUB_013523 |
Construct 5 | PGAPDH-LpPPS-NATR | This study | JPUB_013525 |
Construct 6 | PGAPDH-RtPPS-NATR | This study | JPUB_013527 |
Construct 7 | PTEF1-RtPPS-HYGR | This study | JPUB_013529 |
Construct 8 | PANT-RtPPS-PTEF1-RtPPS-NATR | This study | JPUB_013531 |
Strains | |||
IFO0880 (WT) | Rhodosporidium toruloides strain IFO0880, mating type A2 | NBRC culture collection | |
EIZS1 | IFO0880/PTEF1-RtEIZS-HYGR | This study | JPUB_013534 |
EIZS2 | IFO0880/PGAPDH-RtEIZS-NATR | This study | JPUB_013532 |
EIZS3 | IFO0880/PGAPDH-RtEIZS-NATR/PTEF1-RtEIZS-HYGR | This study | JPUB_013533 |
EIZS4 | IFO0880/PANT-RtEIZS-PTEF1-RtEIZS-NATR | This study | JPUB_013535 |
EIZS5 | IFO0880/PANT-RtEIZS-PTEF1-RtEIZS-NATR/PTEF1-RtEIZS-HYGR | This study | JPUB_013536 |
PPS1 | IFO0880/PTEF1-RtPPS-HYGR | This study | JPUB_013539 |
PPS2 | IFO0880/PGAPDH-RtPPS-NATR | This study | JPUB_013537 |
PPS3 | IFO0880/PGAPDH-RtPPS-NATR/PTEF1-RtPPS-HYGR | This study | JPUB_013538 |
PPS4 | IFO0880/PANT-RtPPS-PTEF1-RtPPS-NATR | This study | JPUB_013540 |
PPS5 | IFO0880/PANT-RtPPS-PTEF1-RtPPS-NATR/PTEF1-RtPPS-HYGR | This study | JPUB_013541 |
GAPDH glyceraldehyde 3-phosphate dehydrogenase, TEF1 translational elongation factor, ANT adenine nucleotide translocase, ScEIZS epi-isozizaene synthase from Streptomyces coelicolor A3(2) (NCBI accession number, WP_011030119.1), RtEIZS epi-isozizaene synthase from S. coelicolor A3(2) codon optimized for R. toruloides, LpPPS prespatane synthase from Laurencia pacifica (ASV63464.1), RtPPS prespatane synthase from L. pacifica codon optimized for R. toruloides, NATR nourseothricin resistance; HYGR, hygromycin B resistance