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. 2020 Nov 12;19:208. doi: 10.1186/s12934-020-01456-4

Table 2.

Genotypes, features and JBEI registry IDs of plasmid constructs and R. toruloides strains investigated in this study

Plasmids Genotypes/features Source/references JBEI registry ID
Construct 1 PGAPDH-ScEIZS-NATR This study JPUB_013517
Construct 2 PGAPDH-RtEIZS-NATR This study JPUB_013519
Construct 3 PTEF1-RtEIZS-HYGR This study JPUB_013521
Construct 4 PANT-RtEIZS-PTEF1-RtEIZS-NATR This study JPUB_013523
Construct 5 PGAPDH-LpPPS-NATR This study JPUB_013525
Construct 6 PGAPDH-RtPPS-NATR This study JPUB_013527
Construct 7 PTEF1-RtPPS-HYGR This study JPUB_013529
Construct 8 PANT-RtPPS-PTEF1-RtPPS-NATR This study JPUB_013531
Strains
IFO0880 (WT) Rhodosporidium toruloides strain IFO0880, mating type A2 NBRC culture collection
EIZS1 IFO0880/PTEF1-RtEIZS-HYGR This study JPUB_013534
EIZS2 IFO0880/PGAPDH-RtEIZS-NATR This study JPUB_013532
EIZS3 IFO0880/PGAPDH-RtEIZS-NATR/PTEF1-RtEIZS-HYGR This study JPUB_013533
EIZS4 IFO0880/PANT-RtEIZS-PTEF1-RtEIZS-NATR This study JPUB_013535
EIZS5 IFO0880/PANT-RtEIZS-PTEF1-RtEIZS-NATR/PTEF1-RtEIZS-HYGR This study JPUB_013536
PPS1 IFO0880/PTEF1-RtPPS-HYGR This study JPUB_013539
PPS2 IFO0880/PGAPDH-RtPPS-NATR This study JPUB_013537
PPS3 IFO0880/PGAPDH-RtPPS-NATR/PTEF1-RtPPS-HYGR This study JPUB_013538
PPS4 IFO0880/PANT-RtPPS-PTEF1-RtPPS-NATR This study JPUB_013540
PPS5 IFO0880/PANT-RtPPS-PTEF1-RtPPS-NATR/PTEF1-RtPPS-HYGR This study JPUB_013541

GAPDH glyceraldehyde 3-phosphate dehydrogenase, TEF1 translational elongation factor, ANT adenine nucleotide translocase, ScEIZS epi-isozizaene synthase from Streptomyces coelicolor A3(2) (NCBI accession number, WP_011030119.1), RtEIZS epi-isozizaene synthase from S. coelicolor A3(2) codon optimized for R. toruloides, LpPPS prespatane synthase from Laurencia pacifica (ASV63464.1), RtPPS prespatane synthase from L. pacifica codon optimized for R. toruloides, NATR nourseothricin resistance; HYGR, hygromycin B resistance