Table 3.
Comparison of pretreatment, enzymatic saccharification, and sugar generation between poplar hydrolysate batches and the sorghum method used previously [26]
| Batch 1 | Batch 2 | Batch 3 | Sundstrom et al. [26] | |
|---|---|---|---|---|
| Pretreatment | ||||
| Biomass loading (%)a | 30 | 25 | 25 | 30 |
| [Ch][Lys]:water | 1:09 | 1:09 | 1:09 | 1:09 |
| Temperature (°C) | 140 | 140 | 140 | 140 |
| Duration (hour)a | 1 | 3 | 3 | 1 |
| Enzymatic saccharification | ||||
| H2SO4 pH adjustment to 5 (v/v) | 50 | 50 | 50 | 50 |
| Solids loading (w/w) | 20 | 20 | 20 | 20 |
| Cellic enzymesa | CTec3/HTec3 | CTec2/HTec2 | CTec3/HTec3 | CTec2/HTec2 |
| Enzyme:water | 9:01 | 9:01 | 9:01 | 9:01 |
| Temperature (°C) | 50 | 50 | 50 | 50 |
| Duration (hour) | 72 | 72 | 72 | 72 |
| Sugar | ||||
| Glucose (g/L) | 46.3 | 50.1 | 77 | – |
| Xylose (g/L) | 14.2 | 16.5 | 26.6 | – |
aRows indicate variation in biomass loading, pretreatment duration, and enzymes