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. 2020 Nov 12;39:240. doi: 10.1186/s13046-020-01731-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Demethylase FTO elevates the expression of MYC by blocking the m6A modification of MYC in CRC cells. a: The mRNA expression of MYC in clinical CRC tissues and non-tumor tissues determined by RT-qPCR (n = 60 for patients in each group). b: The mRNA expression of MYC in CRC cells and HIEC determined by RT-qPCR. c: The protein expression of FTO and MYC in CRC cells after transfection with sh-FTO measured by Western blot analysis. d: The m6A modification level of MYC in CRC cells assessed by Me-RIP assay. e: The binding relationship between FTO and MYC mRNA examined by PAR-CLIP assay; ^*p < 0.05. Measurement data (mean ± standard deviation) between the two groups were compared by paired t test and those among multiple groups were compared by one-way ANOVA with Tukey’s post hoc tests. Cell experiment was independently repeated for three times