Fig. 5.

Demethylase FTO restrains CRC cell proliferation, migration, cell cycle progression and facilitates cell apoptosis through upregulation of MYC. a: The protein expression of FTO and MYC in CRC cells co-transfected with sh-FTO/sh-NC and oe-NC/oe-MYC measured by Western blot analysis. b: The viability of CRC cells co-transfected with sh-FTO/sh-NC and oe-NC/oe-MYC assessed by CCK-8 method. c: Quantitative analysis for cell cycle distribution in CRC cells co-transfected with sh-FTO/sh-NC and oe-NC/oe-MYC assessed by flow cytometry. d: Quantitative analysis for apoptosis rate of CRC cells co-transfected with sh-FTO/sh-NC and oe-NC/oe-MYC evaluated by flow cytometry. e: The expression of proliferation-, cell cycle- and apoptosis-related proteins in CRC cells co-transfected with sh-FTO/sh-NC and oe-NC/oe-MYC, as measured by Western blot analysis. f: The migratory ability of CRC cells co-transfected with sh-FTO/sh-NC and oe-NC/oe-MYC, as measured using scratch test. ^*p < 0.05. Measurement data (mean ± standard deviation) among multiple groups were compared by one-way ANOVA with Tukey’s post hoc tests. Repeated measures ANOVA was used for data comparison between groups at different time points, followed by Bonferroni post hoc test. Cell experiment was independently repeated three times