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. 2012 Jul 11;103(8):1420–1428. doi: 10.1111/j.1349-7006.2012.02347.x

Figure 1.

Figure 1

Zinc finger E‐box binding homeobox 1 (ZEB1) is overexpressed in small cell lung cancer (SCLC) with bone metastasis. Monoclonal mouse anti‐ZEB1 antibodies were used to stain paraffin sections. (A) ZEB1 protein expression in SCLC tissues with bone metastasis and SCLC with no metastasis (400×). (a,b) small cell lung cancer with bone metastasis. (c,d) small cell lung cancer with no metastasis. (B) The results of immunohistochemical staining were evaluated by staining scores as described in the Materials and Methods. B‐M, bone metastatic; N‐M, non‐metastatic. (C) Expression of ZEB1 in SCLC with metastasis (M) and non‐metastatic tissues (N). SCLC tissues with metastasis had higher ZEB1 protein levels than SCLC tissues with no metastasis (P < 0.01). (D) The effect of ZEB1 mRNA expression in non‐metastatic (NM) compared with metastatic (BM) tissues. (E) Expression of ZEB1 protein and (F) mRNA in SBC‐5 cells were significantly higher than those in SBC‐3 or NR8383 cells (P < 0.01 versus SBC‐3 or NR8383 cells).