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. 2012 Jul 11;103(8):1420–1428. doi: 10.1111/j.1349-7006.2012.02347.x

Figure 2.

Figure 2

The effect of lentivirus‐mediated knockdown of zinc finger E‐box binding homeobox 1 (ZEB1) expression in SBC‐5 cells. Lentivirus vectors for the ZEB1 siRNA were constructed and shown to be specific and potent for silencing ZEB1 expression in the SBC‐5 cell line. (A) To observe the infection efficiency of ZEB1 siRNA (ZEB1‐si) and scramble siRNA (Src‐si) by fluorescence microscope. (B) Western blot analysis of ZEB1 protein expression in SBC‐5 cells infected with the lenti‐siRNA/ZEB1 and src‐siRNA. (C) RT‐PCR analysis indicating ZEB1 mRNA levels in SBC‐5 cells transfected with the lenti‐siRNA/ZEB1 vectors or the src‐siRNA vector. The src‐siRNA‐transfected cells were used as a blank control.