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. 2012 Jul 11;103(8):1420–1428. doi: 10.1111/j.1349-7006.2012.02347.x

Figure 3.

Figure 3

Zinc finger E‐box binding homeobox 1 (ZEB1)‐siRNA inhibits cell invasion, migration and expression of PTHrP in vitro. (A) Cell migration was evaluated by counting cells invading through a transwell membrane with an 8‐μm pore size. (B) Results of migration analysis were evaluated as described in the Materials and Methods. *P < 0.01 versus scr‐siRNA treatment. (C) Results of invasion analysis were evaluated as described in the Materials and Methods. *P < 0.01 versus scr‐siRNA treatment. (D) Cell invasion was evaluated by counting cells invading through a Matrigel‐coated transwell membrane with an 8‐μm pore size. Cells attached to the Matrigel were counted under a light microscope. (E) parathyroid hormone‐related protein (PTHrP) was expressed at higher levels in SBC‐5 cells compared to that in SBC‐3 cells, as determined by ELISA. However, there was no obvious change in vascular endothelial growth factor (VEGF) expression. (F) Knockdown of ZEB1 expression resulted in a significant decrease in expression of PTHrP, but there was no obvious change in VEGF expression.