Figure 4.

Zinc finger E‐box binding homeobox 1 (ZEB1)‐siRNA inhibits the number of bone metastases in vivo. Inhibition of bone metastasis by SBC‐5 cells transfected with the lenti‐siRNA/ZEB1 vectors or scr‐siRNA vector in NK‐cell depleted SCID mice. SBC‐5 cells (1 × 10⁶) were intravenously inoculated into NK‐cell depleted SCID mice. After 35 days, evidence of bone metastasis was evaluated using X‐ray photographs. (A) Analysis of SBC‐5 cell lines transfected with ZEB1 knockdown or control vectors. Arrows indicate bone metastasis. (B) Results evaluated the number of bone metastases as described in the Materials and Methods. *P < 0.01 versus scr‐siRNA treatment.