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. 2012 Jul 11;103(8):1420–1428. doi: 10.1111/j.1349-7006.2012.02347.x

Figure 5.

Figure 5

Upregulation of zinc finger E‐box binding homeobox 1 (ZEB1) expression promotes invasion, migration and parathyroid hormone‐related protein (PTHrP) expression in SBC‐3 cells and increases the number of bone metastases in vivo. (A) RT‐PCR of the ZEB1 mRNA from SBC‐3 cells transfected with the ZEB1‐cDNA or control vector. (B) Western blot analysis of ZEB1 protein expression in SBC‐3 cells transfected with the ZEB1‐cDNA or control vector. (C) Results of migration analysis were evaluated as described in the Materials and Methods. *P < 0.01 versus control treatment. (D) Results of invasion analysis were evaluated as described in the Materials and Methods. *P < 0.01 versus control treatment. (E) Upregulation of ZEB1 expression significantly increased the expression of PTHrP, but there was no obvious change in vascular endothelial growth factor (VEGF) expression. (F) Results of animal experiments were evaluated as described in the Materials and Methods. *P < 0.01 versus control treatment. (G) Animals experiments utilizing SBC‐3 cell lines transfected with ZEB1 overexpression or control vectors. Arrows indicate bone metastases.