Figure 6.

Collagen triple helix repeat containing 1 (CTHRC1) mRNA and protein of SGC7901 cells stimulated with transforming growth factor (TGF)‐β1 (10 ng/mL) for the indicated lengths of time as assessed by real‐time PCR (A) and Western blot (B) analyses. Both CTHRC1 mRNA and protein levels increased gradually in response to stimulation with TGF‐β1. Western blot analyses were also carried out with anti‐pSmad2 and anti‐(pan)Smad2 antibodies, and pho‐Smad2 increased in a time‐dependent manner (B).