Figure 4.

Inhibition of intermedin (IMD) induces hepatocellular carcinoma (HCC) cell apoptosis. (A) SK‐Hep‐1 cells were treated with reagent as indicated for 72 h followed by flow cytometry analysis. Numbers in the upper left quadrants of dot plots indicate the percentage of necrotic cells; numbers in the upper right quadrants, late apoptotic cells; numbers in the lower left quadrants, viable cells; numbers in the lower right quadrants, early apoptotic cells. (B) representative immunoblot of apoptotic proteins in SK‐Hep‐1 cells treated with or without IMD antagonist (ant, 1 μg/mL) or antibody (Ab, 1 μg/mL) for 48 h.