Skip to main content
. 2012 Feb 21;103(5):868–874. doi: 10.1111/j.1349-7006.2012.02223.x

Table 1.

Sequences of the primers used in the present study to amplify the 10 fragments for sequencing of the BAP1 coding region

Fragment Target Primer design region Primer sequence
 1 Exon 1–3 Exon 1 Forward: 5′‐CCGTTGTCTGTGTGTGGGACTGAG‐3′
Intron 3 Reverse: 5′‐AGAGCAAGGCTGCTGCTTTCTGTG‐3′
 2 Exon 4 Intron 3 Forward: 5′‐CACCTGAGTGATGACGCAGTGCAA‐3′
Intron 4 Reverse: 5′‐TACCCACTGGATATCTGAGGACAC‐3′
 3 Exon 5 Intron 4 Forward: 5′‐TAGGAGGGTGTCTGAGTCCACTC‐3′
Intron 5 Reverse: 5′‐CAGATCTGCCCAGTTGGCTGTGAG‐3′
 4 Exon 6–7 Intron 5 Forward: 5′‐CCACCCATAGTCCTACCTG‐3′
Intron 7 Reverse: 5′‐AACAGGCCTCCAGCTCATGGTG‐3′
 5 Exon 8 Exon 7 Forward: 5′‐GGCTGAAGGTCTACCCCATTGAC‐3′
Intron 8 Reverse: 5′‐CCAGATTCACCATATGGCCTTGCA‐3′
 6 Exon 9–10 Intron 8 Forward: 5′‐GTGCCTGGCATGTATGGC‐3′
Intron 10 Reverse: 5′‐CCTCCCATGTCAGACATTAG‐3′
 7 Exon 11 Intron 10 Forward: 5′‐TTCTCTGGGAAGTGCTGGTT‐3′
Intron 11 Reverse: 5′‐GGAACCACATGGGAAAATTG‐3′
 8 Exon 12 Intron 11 Forward: 5′‐CAAGGACAGGCCATGGAAC‐3′
Intron 12 Reverse: 5′‐AGGTGCTCAACATTATCTGC‐3′
 9 Exon 13–14 Intron 12 Forward: 5′‐CATTCTGGGTACTGCTGGGT‐3′
Intron 14 Reverse: 5′‐CCACCAATCTTCACACCAAA‐3′
10 Exon 15–17 Intron 14 Forward: 5′‐TCCTTGCCTCTAGCTGCCTAT‐3′
Exon 17 Reverse: 5′‐TACTGGGAAAAGGGGAAGTG‐3′